fig2
![Dichloroacetate enhances the antitumor effect of pirarubicin via regulating the ROS-JNK signaling pathway in liver cancer cells](https://image.oaes.cc/98a83b4e-0267-42f8-9236-95306608c402/3643.fig.2.jpg)
Figure 2. DCA promotes THP-induced apoptosis in liver cancer cells. Hep3B and Huh7 cells were treated with DCA (20 mmol/L), THP (300 nmol/L) or a combination of DCA and THP for 24 h. A: cells were processed for flow cytometry analysis, and the percentage of apoptotic cells was calculated as the percentage of PI and Annexin-V double-positive cells. Data are presented as the mean ± SD from three independent experiments; B: cells were stained with Hoechst 33258 and observed under a fluorescence microscope. The percentage of cells with apoptotic bodies was calculated; C: the activation of PARP was evaluated using Western blotting with α-tubulin as a loading control; D: the relative level of cleaved PARP was calculated. Data are presented as the mean ± SD from three independent experiments. **P < 0.01. DCA: dichloroacetate; THP: pirarubicin; PI: propidium iodide