fig4

Figure 4. HIF-2α silenced CSC neurospheres display differentiation markers. The micrographs depict cryosections of: U87 spheroids (A-D); CSC neurospheres (E-H); and HIF-2α silenced spheroids (I-L). Neurospheres of all categories were stained for stem and differentiated cell markers as indicated. Cells were stained with antibodies to nestin and CD133 for neural stem cell markers, and antibody stains of GFAP, β-tubulin, and MBP were used as markers of differentiated cells. DAPI was the nuclear stain used. HIF: hypoxia inducible factor; CSC: cancer stem cell; GFAP: glial fibrillary acid protein; MBP: myelin basic protein; DAPI: 4',6-diamidino-2-phenylindole