fig3

Figure 3. HIF-2α silencing reduces migration rates of CSCs derived from either U87 cells or BTSC. Cells grown in the presence of DFX were treated with HIF-2α or non-targeting siRNA for 24 h, at which time they were seeded into the top well of a Transwell system and allowed to migrate for 24 h towards medium conditioned by M1 or M2 macrophages or supplemented with LPS or IL-4 as indicated. At least 50 cells per field and 10 fields were counted (10×) of cells that migrated through the Transwell. Normalized cell migration was calculated as the fold increase of cell migration over non-targeting siRNA-treated cells exposed to the same medium conditions. Statistics compare each of the respective medium conditions to U87 cells; *P < 0.05, n = 3. BTSC: brain tumor-derived stem cell; siRNA: short interfering RNA; HIF: hypoxia inducible factor; CSC: cancer stem cell; DFX: deferoxamine mesylate; LPS: lipopolysaccharide; IL-4: interleukin-4