fig1

Figure 1. A, B: HIF-2α expression in CSCs. Representative immunoblots of whole cell lysates are shown. Blots were probed for HIF-2α, and GAPDH was used as a housekeeping gene to normalize for protein loading. C, D: bar graphs obtained by densitometric analysis of Western blot data are shown. Results (mean ± SEM) represent the ratio between HIF-2α and GAPDH levels and are further normalized to the conditions shown in Lane 1 of the corresponding blot; P < 0.05, n = 2 independent experiments. Lanes 3 and 4 in (B, D) received the same HIF-2α siRNA but with different transfection reagents, showing that the CAM lipid is at least as effective as Lipofectamine RNAiMax in this application. siRNA: short interfering RNA; HIF: hypoxia inducible factor; CSC: cancer stem cell; DFX: deferoxamine mesylate; CAM: cationic amphiphilic macromolecule; GAPDH: glyceraldehyde 3-phosphate dehydrogenase