fig4

Figure 4. A: The raw experimental fluorescence data are plotted against time with added DNR (35 μM) at 50 s. After ~700 s, DNR (35 μM) + CsA (5 μM) was added. The fluorescence intensity was greatly enhanced, reaching its maximum peak due to non-competitive inhibition of the ABC transporters by CsA; B: Fluorescence measurement of detection window (dotted black box) are marked to show the location to measure the background signals; C: Fluorescence measurement was conducted within the detection window (dotted black box) to monitor the cell signals. DNR: daunorubicin; CsA: cyclosporine A; ABC: ATP binding cassette