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Figure 2. Expression of (A) MGMT and TfR1; (B) MMR protein hMLH1 in lysates prepared from U373V, U373M, HCT 116 cancer cell lines and MRC-5 fibroblasts. Proteins (50 μg) were separated by PAGE. Monoclonal antibodies were used to detect proteins of interest in addition to GAPDH and Actin to ensure equal loading and detection. Representative cropped blots are shown. Lysates were prepared on ≥ 3 separate occasions