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Figure 2. MiRNA mimic-induced sensitisation to chemotherapeutics in MPM cell lines. A: Transfection with mimics for miR-15a, miR-16 and miR-34a reverses drug resistance in parental and drug resistant cell lines. Cells were transfected with miRNA mimics (at concentrations of 0.5 nmol/L and 1 nmol/L) together with a control (1 nmol/L). After 24 h, MSTO parental cells (blue) together with MSTO-GemR, MSTO-CisR and MSTO-VinoR drug-resistant cell lines (red), were treated with 2-fold serial dilutions of gemcitabine, cisplatin and vinorelbine respectively for 96 h after which cell proliferation was assayed. Drug and mimic treated cells were normalised to mimic treatment alone (mimic or control transfected = 100 %). Data are the mean ± SD of duplicate measurements and are representative of 3 experiments producing similar results; B: Drug IC₅₀ ratios with miRNA mimic transfection in MPM cell lines. The average reductions of chemotherapeutic IC₅₀ values induced by miRNA restoration, across 3 replicate experiments, were depicted as a ratio of the IC₅₀ values from mimic transfected cells compared to those transfected with a control mimic. Data is the average of 3 replicate experiments ± SEM. *P < 0.05 (two-tailed independent samples t-test)