fig1

Figure 1. A: Schematic diagram of the metabolic cycle of THF and the one-carbon (1C) metabolic pathway in folate metabolism, which are regulated by SHMT2, AMT, MTHFD2, ALDH1L2, and MTHFD1L in mitochondria and that of MTHFD1 and SHMT1 in cytoplasm; B: Candidate compound search procedure with in silico molecular docking and a cell-based assay. THF: tetrahydrofolate; SHMT2: serine hydroxymethyltransferase 2; AMT: aminomethyltransferase; MTHFD2: methylenetetrahydrofolate dehydrogenase 2; ALDH1L2: aldehyde dehydrogenase 1 family member L2; MTHFD1L: methylenetetrahydrofolate dehydrogenase 1 like; MTHFD1: methylenetetrahydrofolate dehydrogenase 1