fig3

Figure 3. Binding of nucleotides to NBD1 and NBD2 (A) Asymmetric ATP binding to NBD1 in the inward-open conformation obtained from the crystal structure of mP-gp (PDB:5KOY). The NBDs are represented by an electrostatic potential surface with positive potential shown in blue, negative potential in red. The bound ATP at the nucleotide-binding site of NBD1 is rendered as a ball-and-stick model and is labeled. (B) ATP binding to both NBDs obtained in the structure with open-outward conformation from a cryo-EM study of hP-gp (PDB:6C0V)