fig1

Figure 1. SNX1 depletion promotes efficient ligand-induced EGFR endocytosis in gefitinib-resistant NSCLC cell lines. The 3 NSCLC cell lines PC9 (A), QG56 (B) and A549 (C) cells transfected with siRNA-control (si-Ctrl) or siRNA-SNX1 (si-SNX1) were incubated with Texas red-EGF for the indicated times, and the distribution of the internalized Texas Red-EGF in the LIMPII-positive late endosomes/lysosomes was studied by confocal immunofluorescence microscopy. Superimposed images of Texas red-EGF (red) with LIMPII (green) are shown, and the merged confocal images with a yellow color are indicated by white arrows. Each cell was stained with DAPI (blue) to reveal nuclei. Quantitative analysis was carried out to determine the amounts of LIMPII-positive late endosomes/lysosomes marker that co-localized with the endocytosed Texas red-EGF following 30 min internalization in each cell transfected with si-Ctrl or si-SNX1 (D). Adapted from Nishimura et al.^[41]