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![Reversal of doxorubicin resistance in lung cancer cells by neferine is explained by nuclear factor erythroid-derived 2-like 2 mediated lung resistance protein down regulation](https://image.oaes.cc/111b0db9-132c-45f9-9530-02575fd741de/3424.fig.7.jpg)
Figure 7. Nef and Dox combination induces apoptosis in 3D multicellular spheroids of lung cancer cells. Multicellular spheroids of A549 and A549/Dox cells were generated and treated with Nef/Dox alone or in combination. A: light microscopic images of the spheroids after treatment period (100× magnification); B: line graph showing the percentage (%) of phosphatidyserine externalization using flow cytometric analysis; C: bar graph showing the cell viability of multicellular spheroids. The results in the graph shown are mean ± SEM and *** represents P < 0.01 which are 3 separate experiments performed in triplicate. Nef: Neferine; Dox: Doxorubicin; PS: phosphatidyserine