fig7

Figure 7. Nef and Dox combination induces apoptosis in 3D multicellular spheroids of lung cancer cells. Multicellular spheroids of A549 and A549/Dox cells were generated and treated with Nef/Dox alone or in combination. A: light microscopic images of the spheroids after treatment period (100× magnification); B: line graph showing the percentage (%) of phosphatidyserine externalization using flow cytometric analysis; C: bar graph showing the cell viability of multicellular spheroids. The results in the graph shown are mean ± SEM and *** represents P < 0.01 which are 3 separate experiments performed in triplicate. Nef: Neferine; Dox: Doxorubicin; PS: phosphatidyserine