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Figure 6. NRF2 regulates LRP expression in lung cancer cells treated with Nef and Dox. A-D: representative western blot analysis of A549 and A549/Dox cells treated with Dox alone, Nef alone and Nef and Dox combination and densitometric analysis are shown for the proteins p-NRF2 and HO-1; E, F: A549 or A549/Dox cells were knocked out for NRF2 using siNRf2 and treated with Dox in combination with Nef. Cell lysates were analyzed for LRP expression and a representative image and densitometric analysis were shown. GAPDH served as internal control for western blots. The results in the graph shown are mean ± SEM and *** represents P < 0.01 which are 3 separate experiments performed in triplicate. Nef: Neferine; Dox: Doxorubicin; NRF2: nuclear factor erythroid-derived 2-like 2; LRP: lung resistance protein; GAPDH: Glyceraldehyde 3-phosphate dehydrogenase