fig3

Figure 3. Intracellular DOX accumulation was potentiated by Nef. A549 or A549/Dox cells were treated with 10 μM Nef and/or 3 μM Dox and the intracellular dox was assessed as mentioned in materials and methods: A: fluorescent microscope (magnification 400×); B: flow cytometry. Results shown are are three separate experiments performed in triplicate. Nef: Neferine; Dox: Doxorubicin