fig2

Figure 2. Nef-Dox combination therapy induces apoptosis in treated lung cancer cells. Line graph showing the percentage (%) of: A: phosphatidyserine externalization using flow cytometric analysis; B: loss of mitochondrial membrane potential using fluorescence spectrophotometer; C, D: representative western blot analysis of A549 and A549/Dox cells treated with Dox alone, Nef alone and Nef and Dox combination and densitometric analysis are shown. Bax, Bcl2, caspase 3 and cleaved caspase 3 protein expressions were probed. GAPDH served as the loading control. PS: phosphatidylserine ; Nef: Neferine; Dox: Doxorubicin; GAPDH: Glyceraldehyde 3-phosphate dehydrogenase