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Figure 2. Augmented expression of WT1 in cytogenetically distinct B-lineage acute lymphoblastic leukemia (ALL) subsets. Increased levels of WT1 expression in ALL subsets compared to normal samples from the Microarray Innovations in Leukemia (MILE) Study. Gene expression profiling data from the MILE Study were interrogated for differences in WT1 expression levels. A: DQN3-normalized expression levels for the WT1 probeset, 206067_s_at in ALL subsets are depicted using mean and standard error values presented in the bar chart (± SEM). Primary leukemia cells from MLL-R⁺ B-lineage ALL patients exhibited the highest expression levels for WT1. Sample sizes for t(9;22), t(8;14), t(12;21), t(1;19), MLL-R, Hyperdiploid, Non-leukemic healthy BM were 122, 13, 58, 36, 70, 40 and 74 respectively; B: one way ANOVA was performed across 7 subgroups of B-lineage ALL cases in the MILE study and statistical significance was assessed utilizing linear contrasts of each subgroup vs. nonleukemic control samples, and for MLL-R subgroup vs. the other B-lineage ALL subgroups combined. Depicted are the mean values fold differences in expression and the corresponding P-values