fig4

Figure 4. Expression of CD22ΔE12 signature genes in Ph-like ALL. In total 370 Ph-like ALL cases were identified using a set of 110 gene probeset gene expression signature developed utilizing the Affymetrix U133 plus 2.0 microarray platform, as described in the "METHODS". The RMA-normalized gene expression values for leukemia cells obtained from these 370 Ph-like ALL patients [GSE11877 (n = 99), GSE13159 (n = 216), GSE13351(n = 55)] were log₂ transformed and mean-centered to the average value for leukemia cells from 225 BCR-ABL^- BPL patients (GSE1187, GSE13159, GSE13351; Ph-like ALL, MLL-R⁺ and t[1;19]/E2A-PBX1⁺patients excluded). To determine the differential expression of each of the leading-edge genes of the CD22ΔE12 transcriptome, linear contrasts were performed for the mean-centered values. Heat map depicts up and down regulated transcripts for mean-centered expression values and clustered according to average distance metric