fig1

Figure 1. Unique signature transcriptome of CD22ΔE12 transgenic (Tg) BPL cells. (A) Depicted is a one-way hierarchical cluster figure that illustrates gene expression differences between BPL cells from CD22ΔE12-Tg mice and normal splenocytes from healthy WT mice, BPL cells from BCR-ABL Tg mice or BPL cells from Eµ-MYC Tg mice; (B) Depicted is a two-way agglomerative hierarchical cluster figure whereby the dendrograms were joined by the average distance metric. The heat map depicts the NES scores for 32 Reactome pathway gene sets related to DNA replication, transcription, translation and cell cycle regulation that showed significant upregulation (P < 0.01) for all three comparisons of CD22ΔE12-Tg vs. WT, CD22ΔE12-Tg vs. Eµ-MYC Tg and CD22ΔE12-Tg vs. BCR-ABL Tg. A sub-cluster of highly significant enrichment of 7 large Reactome pathways (URN 2, 3, 4, 5, 7, 8, and 9 - see Supplementary Table 1) (P < 0.001 for all comparisons) was revealed in this representation highlighting the role of CD22ΔE12 in cellular re-programming