fig4

Figure 4. Ras expression by T24 cells as visualized by immunostaining. (A) Untreated cells; (B and C) T24 cells treated with 3 and 30 μg/mL MMC after 24 h. The intensity of staining increased after treatment as compared with untreated cells; (D, E, and F) cells treated with 0.3, 3, and 30 μg/mL MMC after 72 h, respectively. The staining intensity showed progressive reduction with increasing MMC dose to reach the minimum after treatment with 100 μg/mL (not shown); (G, H, and I) cells treated with negative control, 50, and 100 nmol/L ras siRNA after 72 h, respectively. The cells showed little intensity of staining when compared to negative control with the least intensity at 100 nmol/L; (J and K) cells treated with 0.3 and 3 μg/mL MMC, respectively, combined with 50 nmol/L ras siRNA and cultured for 72 h; (L) cells treated with 3 μg/mL MMC combined with 100 nmol/L ras siRNA and cultured for 72 h. The cells undergoing apoptosis or mitosis (arrows) were more intensely stained compared with other cells (400×). MMC: mitomycin C