fig9

Figure 9. Sequence-dependent abnormal aggregation of human ataxin-3(22Q) takes place in SH-SY5Y cells. (A) Wild-type ataxin-3(22Q) and (B) ataxin-3(22Q)-F74P over-expressed in SH-SY5Y neuroblastoma cells formed aggregates in the cells. In contrast, no aggregates were observed in either (C) ataxin-3(22Q)-I80P or (D) ataxin-3(22Q)-A83P. SH-SY5Y cells were stained with 0.1% ThS, coimmunostained with primary monoclonal antibodies Anti-FLAG and secondary Alexa Fluo-546, and visualized by confocal microscopy. The scale bar represents 10 m. ThS: Thioflavin S.