fig5

Figure 5. The fibril-forming motif of the Josephin domain was identified based on CD measurements of ataxin-3(22Q) and proline mutations. Far-UV CD spectra of the following mutations: (A) ataxin-3(22Q); (B) ataxin-3(22Q)/F74P; (C) ataxin-3(22Q)/S81P; (D) ataxin-3(22Q)/N82P; (E) ataxin-3(22Q)/L89P; and (F) ataxin-3(22Q)/I92P. The experimental conditions are the same as those in Figure 4. CD: Circular dichroism; far-UV: far-ultraviolet.