fig4
![Fibril-forming motif of non-expanded ataxin-3 revealed by scanning proline mutagenesis](https://image.oaes.cc/b5720fb6-1f82-4c27-9f66-82a2d36fb250/and3015.fig.4.jpg)
Figure 4. Different mutations have different influences on ataxin-3(22Q) fibril morphology based on TEM measurements of the Ataxin-3(22Q) and proline mutations. Negative-stain transmission electron micrographs of the following mutants: (A) ataxin-3(22Q); (B) ataxin-3(22Q)/F74P; (C) ataxin-3(22Q)/S81P; (D) ataxin-3(22Q)/N82P; (E) ataxin-3(22Q)/L89P; (F) and ataxin-3(22Q)/I92P. The concentration of ataxin-3(22Q) and mutations was 50 M, and 10 mM TBS buffer (pH 7.4) containing 5 mM DTT was used. The assays were carried out at 37 °C, and the observation time was 3 days. The scale bars represent 200 nm. TEM: Transmission electron microscopy; DTT: dithiothreitol.