fig3

Figure 3. Different mutations have different influences on ataxin-3(22Q) based on time-dependent SDS-PAGE analysis. 50 µM ataxin-3(22Q) or proline mutations were incubated in 10 mM TBS buffer (pH 7.4) at 37 °C. Aliquots were taken at 0, 1, 2, 3, 4, 5, 6, and 7 days, respectively, and then incubated with TBS buffer containing 1% sarkosyl followed by centrifuging at 150,000 × g for 30 min. Pellets were resuspended with sample buffer containing 5% 2-mercaptoethanol and subjected to 15% SDS-PAGE. Gels were stained with Coomassie Blue. TBS: Tris-buffered saline.