fig2

Figure 2. Different mutations have different influences on ataxin-3(22Q). Kinetic curves for the aggregation of ataxin-3(22Q) (black), F74P (red), F75P (green), S76P (blue), I77P (magenta), I80P (wine), A83P (navy), and V86P (pink), monitored by ThT fluorescence. The concentration of ataxin-3(22Q) and mutations was 50 M, and 10 mM TBS buffer (pH 7.4) containing 5 mM DTT was used. The assays were carried out at 37 °C, and the observation time was 168 h. DTT: Dithiothreitol.