fig7

Figure 7. Formation of p150^Glued-positive protein aggregates in midbrain DA neurons of G59S Tg mice. (A) Immunofluorescent images show the staining of p150^Glued and p135+ (green) and TH (red) in midbrain coronal sections of 24-month-old nTg, WT Tg, G59S Tg, and G71R Tg mice. Scale bar: 20 μm. Arrows point to proteins aggregates of p150^Glued and p135+ in the soma and neurites of DA neurons from G59S Tg mice. (B) Bar graph estimates the percentage of TH-positive neurons with proteins aggregates of p150^Glued and p135+ in the midbrain of 2-, 6-, 12-, and 24-month-old nTg, WT Tg, G59S Tg, and G71R Tg mice (at each time point, n = 4 animals per genotype and 5 sections per animal). Aggregates were defined as puncta ≥ 1 μm². Data are presented as mean ± SEM. At each time point, one-way ANOVA with Dunnett’s multiple comparisons test was used for statistical analysis (the mean of each genotype was compared with the mean of nTg). ^****P < 0.0001. ns: Not significant. TH: Tyrosine hydroxylase.