fig4

Figure 4. Robust degeneration of DA axon terminals in the striatum of G59S Tg and G71R Tg mice. (A) Representative images show TH staining in the dorsal striatum coronal sections of 24-month-old nTg, WT Tg, G59S Tg, and G71R Tg mice. Scale bar: 10 μm. Arrows point to swellings of TH-positive DA axon terminals in the striatum of G59S Tg and G71R Tg mice. (B) Bar graph estimates the area fraction of TH-positive DA axon terminals in the dorsal striatum of 2-, 6-, 12-, and 24-month-old nTg, WT Tg, G59S Tg, and G71R Tg mice (at each time point, n = 4 animals per genotype and 10 sections per animal). Data are presented as mean ± SEM. At each time point, one-way ANOVA with Dunnett’s multiple comparisons test was used for statistical analysis (the mean of each genotype was compared with the mean of nTg). ^**P = 0.0018, ^****P < 0.0001. ns: Not significant. TH: Tyrosine hydroxylase.