fig1

Figure 1. Selective expression of wild-type or mutant human p150^Glued in mouse midbrain DA neurons. (A) The schematic diagram depicts the generation of transgenic mice with target expression of human p150^Glued in midbrain DA neurons by crossing Pitx3-IRE2-tTA and tetO-human DCTN1 mice. (B) Immunofluorescent images show the staining of p150^Glued (green) and TH (red) in the coronal midbrain sections of one-month-old nTg and WT Tg mice. DA neurons were visualized by TH staining. Scale bar: 200 μm. (C, D) Western blots show the expression of human p150^Glued, total (human and mouse) p150^Glued, p135+, DCTN4, p50, and ARP1 in the midbrain homogenates of one-month-old nTg, WT Tg, G59S Tg, and G71R Tg mice. β-actin and TH were used as the loading control. The bar graph estimates the protein level of dynactin subunits normalized with β-actin (n = 4 per genotype). Data are presented as mean ± SEM. One-way ANOVA with Dunnett’s multiple comparisons test was used for statistical analysis (the mean of each genotype was compared with the mean of nTg). ^**P < 0.01, ^***P < 0.001, ^****P < 0.0001.