fig6

Figure 6. Semiquantitative analysis of Aβ₄₀ and Aβ₄₂ oligomers, Aβ plaques, and p-Tau in neurologically intact young (1-5-years), middle (6-10-years), and old (11-16-years) age groups of dogs. PRIOAD12 (Aβ₄₀ oligomers), PRIOAD13 (Aβ₄₂ oligomers), 4G8 (Aβp), and AT8 (p-Tau) fluorescence immunoreactivity and intensities were examined and quantified at 40× magnification under the fluorescence microscope. Semiquantitative analyses were compared with the three different age groups. A large amount of Aβ₄₀ and Aβ₄₂ oligomers were found in young dogs, which decreased in middle age group, then finally, an upward trend was noticed in older dogs. In comparison, Aβp were completely absent in young dogs, and then moderately found in middle, and large amounts in old age groups. Finally, p-Tau deposits were not found in young and middle age groups of dogs, whereas old dogs exhibited a considerable amount of p-Tau.