fig1

Figure 1. Photomicrographs of the microscopic lesions in the canine retina. The retina in a 12-year-old German shepherd dog. Eosinophilic deposits (green arrows) were observed in the ganglion cell layer (GCL) and inner nuclear layer (INL). Representative of 10 cases of old-aged dogs. Hematoxylin and eosin (H&E) 40×. (B) Higher magnification of the deposits in image (A) (green arrowhead) in the GCL. H&E 100×. (C) Retina of a 3-year-old German shepherd dog. Immunohistochemical staining with A11 anti-Aβo IgG antibody exhibited cytoplasmic Aβo depositions in the GCL and INL (green arrows). Representative of 10 cases of young age group. IHC 40×. (D) Higher magnification of deposits from image (C) in the GCL (green arrowheads). IHC 100×. (E) Retina of a 12-year-old German shepherd mix dog. Immunohistochemical staining with 4G8 anti-Aβp IgG antibody exhibited extracellular Aβ aggregates in the GCL and INL (green arrows). IHC 40×. (F) Higher magnification of deposit from image (E) in the GCL (green arrowhead). (G and H) Representative retinal sections derived from dogs of all age groups stained with secondary antibodies with the omission of the primary antibody did not show any depositions (40× and 100×, respectively). The photomicrographs were taken from the peripheral region of the retina - away from the optic disc. Representative of all 30 dogs examined. IHC 100×. IHC: Immunohistochemistry.