fig2

Figure 2. Molecular formats of the earliest HBV-host and WHV-host genomic junctions: (A) Examples of the HBV-host DNA fusions formed (left) by the NHEJ or (right) by MHMOJ detected between 30 min and 24 h post-infection in hepatocyte-like HepG2-NTCP-C4 clone cells or HepaRG cells; (B) Examples of the WHV-host genomic merges created by NHEJ detected (top left) in woodchuck liver biopsy obtained at 1 h post-infection and (bottom left and right) in woodchucks WCM260 hepatocytes at 15 min and 1 h post-infection. Magnifying glasses at the top of each panel show blown-up regions representing virus-host DNA junctions created by NHEJ or MHMOJ. HBV sequences are depicted as continuous lines, while host sequences are marked by dashed lines. HBV: hepatitis B virus; WHV: woodchuck hepatitis virus; MHMOJ: micro-homology-mediated-overlapping-joining; NHEJ: non-homologous end joining; p.i.: post-infection; SINE: short interspersed nuclear element; NBPF: neuroblastoma breakpoint family member; LINE-1: long interspersed nuclear element; NTM: neurotrimin; THE1B-LTR: mammalian apparent LTR retrotransposon; RunX1: runt-related transcription factor-1; MAML-2: mastermind-like 2; host-seq: unidentified woodchuck genomic sequence.