fig4

Figure 4. Functional characterization of dimethylbromobenzene-cysteine p53-MDM2/MDMX inhibitor. A-C: dose-dependent growth inhibition of HCT116 p53^+/+ (A), HCT116 p53^+/+ (B) and SK-hep-1 (C) cells upon various treatments as determined by the MTT assay to monitor the pesticide effects. Three cell lines were plated in 96-well plates at a density of 2,500 cells/well (100 μL). After 24 h, cells were treated with drug sample at the indicated concentrations and times in FBS-free mediums, respectively. The in vitro cytotoxicity was then measured by using a standard MTT (Thermo Fisher scientific) assay after 72 h drug treatment. (mean ± SD, n = 4); D-F: apoptosis levels measured by FACS in three cell lines treated with SP0, SP1 SP2 and SP3 for 48 h incubation at concentration of 50 μmol/L; G-I: the average means of the apoptosis calculated three independent experiments like D-F. P values were calculated by t-test (*P < 0.05; **P < 0.01; ***P < 0.001)