fig1

Figure 1. Schematic illustrating the production of personalized HBV-specific TCR redirected T cells. A: PBMC isolation from HCC patients; B: activation and expansion of αβ TCR T cells for modification; C: transduce activated T cells with viral vectors encoding HBV-specific TCRs; D: electroporate activated T cells with in vitro transcribed mRNA; E: TCR-T cells engineered through viral transduction has the gene encoding the TCR integrated into the genome while electroporation only results in the translation of the introduced mRNA; F: analysis of the expression kinetics and function of HBV-specific TCR-T cells by tetramer staining and immune assays; G: adoptive transfer of autologous HBV-specific TCR-T cells back into the HBV-HCC patient; H: cytolysis of HBV expressing hepatocyte or HCC cells. HBV: hepatitis B virus; TCR: T-cell receptor; PBMC: peripheral blood mononuclear cell; HCC: hepatocellular carcinoma