fig1

Figure 1. Volcano plots of the microRNAs (miRNAs) with significant (P < 0.001) over 2-fold expression changes in hepatocellular carcinoma (HCC) tumor compared to nontumor tissues. (A) There were more downregulated miRNAs aberrantly expressed in HCC tumor tissue with only 3 miRNAs significantly up-regulated if using 3 endogenous controls (U6 snRNA, RNU44 and RNU48) as the normalize; (B) Expression of 26 miRNAs (17 upregulated and 9 downregulated) significantly differed between tumor and non-tumor tissues; (C) Seventeen miRNAs were aberrantly expressed in HCC tumor tissue using 2 stable miRNAs (miR-30c and miR-30b) identified by geNorm tool as the normalize; (D) Twenty miRNAs were significantly deregulated in HCC tumor tissues using 2 stable miRNAs (miR-30c and miR-126) identified by NormFinder tool as the normalizer. The overall expression patterns of miRNAs in (B, C and D) were similar but different from (A) with many more down-regulated miRNAs