fig8
Figure 8. Effects of KIF3A on the proliferation and migration of HCC. (A) Western blot analysis was used to analyze KIF3A expression in LX-2 and HepG2 cells. (B) The knockdown efficiency of KIF3A in HepG2 cells was detected by real-time PCR and Western blotting. (C) CCK-8 assay to detect the proliferation of HepG2 cells after the knockdown of KIF3A. (D and d) A plate colony formation assay was used to detect the colony formation ability of cells after the knockdown of KIF3A. (E and e) BeyoClick™ EdU-488 assay to detect cellular DNA synthesis after the knockdown of KIF3A. (F and f) The migration ability of cells after KIF3A knockdown was detected by a scratch test. (G and g) The invasion ability of cells after KIF3A knockdown was detected by Transwell assay (*P < 0.05, **P< 0.01,