fig8

Expression characteristics, immune signatures, and prognostic value of the KIF family members identified across cancers

Figure 8. Effects of KIF3A on the proliferation and migration of HCC. (A) Western blot analysis was used to analyze KIF3A expression in LX-2 and HepG2 cells. (B) The knockdown efficiency of KIF3A in HepG2 cells was detected by real-time PCR and Western blotting. (C) CCK-8 assay to detect the proliferation of HepG2 cells after the knockdown of KIF3A. (D and d) A plate colony formation assay was used to detect the colony formation ability of cells after the knockdown of KIF3A. (E and e) BeyoClick™ EdU-488 assay to detect cellular DNA synthesis after the knockdown of KIF3A. (F and f) The migration ability of cells after KIF3A knockdown was detected by a scratch test. (G and g) The invasion ability of cells after KIF3A knockdown was detected by Transwell assay (*P < 0.05, **P< 0.01, ***P < 0.001).

Journal of Cancer Metastasis and Treatment
ISSN 2454-2857 (Online) 2394-4722 (Print)

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