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Figure 3. EEF1A2 knockdown suppresses proliferation, migration, and invasion in lung cancer cells. (A) EEF1A2 expression levels in lung cancer cell lines were measured using RT-qPCR and WB analyses. Significance levels are indicated: ***P < 0.001, ****P < 0.0001; ns denotes no significant difference compared to HBE cells. (B) PC-9 and SPCA1 cells were transfected with shEEF1A2 lentivirus (KD-EEF1A2) or control lentivirus (KD-NC), and EEF1A2 expression was evaluated via RT-qPCR and WB. (C) The proliferation of PC-9 and SPCA1 cells transfected with KD-NC or KD-EEF1A2 was assessed using the CCK-8 assay. Statistical significance is represented as *P < 0.05, **P < 0.01, ***P < 0.001; ns indicates no significant difference compared to the KD-NC group. (D) Western blot analysis was performed to detect cyclin D1 and p21 protein levels in PC-9 and SPCA1 cells after transfection with KD-NC or KD-EEF1A2. (E) Wound healing assays were conducted on PC-9 and SPCA1 cells transfected with KD-NC or KD-EEF1A2 to evaluate their migratory capacity. (F and G) Transwell migration and invasion assays were carried out on PC-9 and SPCA1 cells following transfection with KD-NC or KD-EEF1A2. Statistical significance is indicated by *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 compared to the KD-NC group.