fig5

Figure 5. (A) ROS were detected by RNO-ID using Cu-Cy aqueous solution (0.2 mg/mL) irradiated with different MWs of different power for 5 min. (B) Cu-Cy aqueous solution (0.2 mg/mL) irradiated with MW at 20 W for different times was detected by the RNO-ID method. (C) A comparison of SOSG fluorescence intensity with different Cu-Cy concentrations after MW irradiation. (D) A live/dead staining image of HCT15 cells treated with Cu-Cy and MW irradiation (20 W, 3 min). (E) MW-induced colony formation in HCT15 cells. (F) A calculation was made to determine the average number of clones. (G) Experiment images showing each group’s results using the xenograft model. (H) Tumor mass changes. (I) Tumor volume changes. (J) An illustration of how Cu-Cy-mediated dynamic microwave therapy (MWDT) induces ferroptosis in colorectal cancer cells. (K) Western blot assay of GPX4 expression. (L) FCM assay of cellular LPO with BODIPY-C11 probe detection. (M) FCM analysis of relative fluorescence intensity of HCT15 stained with the C11 BODIPY probe. Scale bar: 50 μm; *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001. Reprinted from ref.^[131] with permission. Copyright 2022, Elsevier.