fig7
From: The contribution of proteoglycans to heterogeneity and temozolomide resistance of glioblastoma cells
Figure 7. TMZ effects on the PGs expression and GAG content in the GB cell lines. (A and B) Expression of PG core proteins - CD44 and others (A) and all PGs except CD44 (B). RT-PCR analysis. (C) Immunofluorescence analysis for NG2/CSPG4 core protein and CS and HS content. Representative microphotographs of immunostaining with anti-NG2/CSPG4, anti-CS, and anti-HS antibodies (AlexaFluor 488). Magnification ×200, the scale bar for the micrographs is 100 µm. (D) Quantitative analysis - average fluorescence intensity calculated using MeanGrayValue (ImageJ). ANOVA + Fisher’s LSD test. CS: chondroitin sulfate; HS: heparan sulfate. * P < 0.05.
