fig3
From: The contribution of proteoglycans to heterogeneity and temozolomide resistance of glioblastoma cells
Figure 3. Immunofluorescence analysis for NG2/CSPG4 core protein and CS and HS content in human GB cell lines. (A) Representative microphotographs of immunostaining with anti-NG2, anti-CS, and anti-HS antibodies (Alexa Fluor 488). Magnification ×200, the scale bar for the micrographs is 100 µm. (B) Quantification of the NG2/CSPG4 protein, CS and HS content in the cells -average fluorescence intensity calculated using MeanGrayValue (ImageJ). ANOVA + Fisher’s LSD test. CS: chondroitin sulfate; HS: heparan sulfate.
