fig3
Figure 3. NSCLC cell proliferation, invasion, and glycolysis were suppressed by ZNF148 inhibition. (A) Transfections by si-ZNF148 or the control were performed on A549, PC9, and H1299 cells. P values represent the comparison between si-ZNF148 and si-NC in each cell line. (B) Cells were transfected with si-ZNF148 or the corresponding control and then cell growth was explored by CCK-8. P values represent the comparison between si-ZNF148 and si-NC in each cell line. (C) Cell growth was confirmed by colony formation analysis after transfection with si-ZNF148 or the corresponding control. (D) Statistical graph of colonies. P values represent the comparison between si-ZNF148 and si-NC in each cell line. (E) Transwell assays of A549, PC9, and H1299 cells after transfection with si-ZNF148 or the corresponding control. (F) The number of infiltrated cells was counted. P values represent the comparison between si-ZNF148 and si-NC in each cell line. (G) Glucose consumption in A549, PC9, and H1299 cells was determined after transfection with si-ZNF148 or the corresponding control. P values represent the comparison between si-ZNF148 and si-NC in each cell line. (H) Lactate production in A549, PC9, and H1299 cells was determined after transfection with si-ZNF148 or the corresponding control. P values represent the comparison between si-ZNF148 and si-NC in each cell line. (I) A549, PC9, and H1299 cells were transfected with si-ZNF148 or the corresponding control and then ATP/ADP ratios determined. P values represent the comparison between si-ZNF148 and si-NC in each cell line. (J) Relative 2-NBDG uptake in A549, PC9, and H1299 cells was determined after transfection with si-ZNF148 or the corresponding control. P values represent the comparison between si-ZNF148 and si-NC in each cell line. (K) Following si-ZNF148 transfection or control, immunofluorescence was used to determine GLUT1 and GLUT4 levels in A549, PC9 along with H1299 cells.
