fig2

Figure 2. ZNF148 was overexpressed and could upregulate the expression of PFKP in lung cancer. (A) The potential ZNF148 binding sites within the promoter of the PFKP gene with a homology higher than 90% from JASPAR analysis are shown. (B) ChIP assay was performed with a ZNF148 antibody and analyzed with qPCR as a percent of the input. P1-P5 were the five ZNF148 potential binding sites within the promoter of PFKP. P values represent the comparison between IgG and P1-P5. (C) The expression of PFKP after transfection with si-ZNF148 or overexpression of ZNF148 and relative controls. P values represent the comparison between si-ZNF148 or si-NC and ZNF148 and NC. (D) ZNF148 levels were measured using qRT-PCR. P values represent the comparison between the human normal lung cell line Beas2b and each NSCLC cell line. (E) ZNF148 levels of 40 paired NSCLC samples (Tumor) along with adjacent normal samples (Normal). P values represent the comparison between tumor and normal tissues. **P < 0.01.