fig3

Figure 3. Typical overlaying histograms of autofluorescence (left peak) and fluorescence of MCF-7 mammosphere cells stained with fluorescently labeled antibodies to histone γH2AX (right peak): (A) control mammosphere cells; (B) mammosphere cells 1 h after γ-irradiation at a dose of 2 Gy; (C) mammosphere cells 1 h after γ,n-irradiation at a dose 1 Gy; and (D) the relative level of histone γH2AX calculated from the median fluorescence intensity (MFI) data for the M region which corresponds to γH2AX-positive mammosphere cells with high MFI values. The numbers on the histograms indicate the percentage of positive cells and MFI in the M region. *Differences from the control are statistically significant, P < 0.05. Experiments were repeated five times.