fig4

Figure 4. Cytokine secretion in the supernatants of in vitro cocultures of A549 and PBMCs from healthy donors, with (orange) and without (blue) treatment with human neutrophil peptide1 (HNP1). The results are presented as means ± SEM of the different donors (n = 4; duplicate cultures/donor). *Significant differences (P ≤ 0.05) were analyzed using the Mann-Whitney U test, performed in R Software. (A) Multiplex ELISA analysis performed with U-PLEX (MSD) on the supernatants of the same cocultures, as described earlier, and an additional donor. (B) ELISA analysis performed on the supernatants of A549/PBMC cocultures of four other donors.