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![Bio-fabrication of liver cancer stem cells and testing of gemcitabine](https://image.oaes.cc/a1268d3b-f6e5-4e63-8a9a-f7ae65883f46/5671.fig.1.jpg)
Figure 1. Experimental design (2 × 104 Huh7 cells and alginate solution were mixed and placed in the microfluidic syringe pump. The microfluidic syringe pump was used to inject the mixture at a flow rate of 20 μL/min into a 24-well plate. After polymerizing the alginate with 0.1 M CaCl2, the beads were washed with PBS and cultured using DMEM medium. The cell viability, IL-6 secretion, CD90, and AFP levels of the Huh7 cells encapsulated in alginate were examined for 12 days with/without gemcitabine).