fig1

Figure 1. Experimental design (2 × 10⁴ Huh7 cells and alginate solution were mixed and placed in the microfluidic syringe pump. The microfluidic syringe pump was used to inject the mixture at a flow rate of 20 μL/min into a 24-well plate. After polymerizing the alginate with 0.1 M CaCl₂, the beads were washed with PBS and cultured using DMEM medium. The cell viability, IL-6 secretion, CD90, and AFP levels of the Huh7 cells encapsulated in alginate were examined for 12 days with/without gemcitabine).