fig2
![The antiangiogenic phloroglucinol hyperforin inhibits the secretion of proMMP-2, proMMP-9 and VEGF-A during apoptosis of primary acute myeloid leukemia cells](https://image.oaes.cc/610a56a1-91e6-453c-b38d-bcd4b9d37a4c/4075.fig.2.jpg)
Figure 2. Expression of MMP-2, MMP-9, and VEGF-A in primary AML cells. (A) PCR analyses of MMP-2/9 and VEGF-A transcripts. Samples were standardized for total cDNA content by assessing the presence of identical amounts of β2-microglobulin transcripts. (B) Expression profiles of MMP-2/9 (pro and active forms) were analyzed by zymography using 7.5% (w/v) SDS-polyacrylamide gels containing 0.1% gelatin (w/v) in the 72-h conditioned media (supernatant) from AML cells. The control medium was an FCS-supplemented culture medium alone incubated under the same conditions; the culture supernatant from U937 (M5) cells was used as a positive control for proMMP-2/9 and active MMP-9 proteins. The assay’s sensitivity for MMP-2/9 gelatinolytic activity was