fig3

Figure 3. The absence of RANBP9 causes a marked reduction of p53 levels in NSCLC cells subject to genotoxic stress. Two independent A549 RANBP9 WT controls and RANBP9 KO clones were exposed to 10 μmol/L CDDP for 24 h. WB shows that RANBP9 KO cells have a severe reduction of total p53 levels. This is likely due to the blunted p53 phosphorylation of Serine 15, which is a target of the ATM kinase (as shown in Palmieri et al.^[56], 2016 and Tessari et al.^[50], 2018). RANBP9: RAN binding protein 9; NSCLC: non-small cell lung cancer; KO: knockout