fig6

Figure 6. Re-expression of wt-EGFR fully restores EGF-driven cell motility and lung metastasis formation. A: immunostaining with a specific mouse monoclonal antibody against human EGFR confirmed the ectopic expression of EGFR in S178A cells; paxillinS178A (green) and human EGFR (red). Scale bar is 10 μm; B: trajectories of the movements of S178A and S178AEGFR cells are plotted in (a) and (b) respectively (X and Y are in μm). Protein dynamics was quantified with spot bleaching technique and the fluorescence recovery after 30 sec was slightly faster upon EGF stimulation, * P < 0.05. (c); C: two-photon imaging of GFP-positive lung metastases, scale bar is 100 μm. (a). There was no significant difference in tumor weight of S178A (n = 13) and S178AEGFR (n = 13) groups (b); D: H&E staining of lung slices of S178A and S178AEGFR groups (a). Number of GFP-positive metastases counted in the fresh lungs at time of sacrifice is shown (b)