fig3

Figure 3. Representative images of Western blots performed on five samples of metastatic melanoma to the brain. PRR was detected in all five samples at 40 kDa (A). ACE was detected in all five samples at 190 kDa, with multiple lower bands consistent with protein degradation (B). ATIIR1 was detected at 45 kDa in two samples and at 28 kDa in all five samples (C). ATIIR2 was present at 50 kDa in all five samples (H). Positive controls were mouse brain extract for PRR (A), mouse lung protein extract for ACE (B), mouse brain for ATIIR1 (C), PC3 cell lysate for ATIIR2 (D). Negative controls were mouse kidney for PRR (A), human tonsil for ACE (B), mouse kidney for ATIIR1 (C), and NTERA2 for ATIIR2 (D). PRR: pro-renin receptor; ATIIR1: angiotensin II receptor 1; ATIIR2: angiotensin II receptor 2; ACE: angiotensin converting enzyme