fig7

Figure 7. Schematic representation of the effects of co-culture with embryonic stem cell (ESC)-microstrands on expression of key signaling pathway molecules in MDA-MB-231 breast cancer cells (BCCs). Left Panel: BCC alone; Right Panel: co-cultured breast cancer cells with ESC-microstrands. Changes in protein and mRNA expression after co-culture with ESC-microstrands indicated inhibition of the canonical Wnt/β-catenin (above the dash line) and EGFR (below the dash line) signaling pathways. Co-culture with ESC-microstrands causes NKD2 up-regulation at both the mRNA and protein levels, and this coincides with dual signaling pathway inhibition. It suggested restored ability of NKD2 to inhibit the canonical Wnt/β-catenin signaling pathway and reduced ability to promote the EGFR signaling pathway following co-culture