fig1

Figure 1. A: AF1q expression status in breast cancer cell lines (1: MDA-MB-231, 2: MDA-MB-231-luc2-LN); B: validation of relative expression of gene obtained from RNA-seq by qPCR. qPCR analyses were performed as described in the method section using randomly selected 20 genes (upregulated genes 10 and downregulated genes 10). Relative expression values are presented as an average ± SD of three biological replicates; C: significantly expressed genes. 1,485 genes are significantly expressed in both cell lines with three biological replicates, respectively. Among those, transcripts of 762 genes increased in MDA-MB-231LN cell line and those of 723 genes decreased