fig4

Figure 4. Western blot images of total protein extracted from snap-frozen samples of liver metastasis from colon adenocarcinoma (LMCA) from three patients demonstrating the presence of pro-renin receptor (PRR) in all three samples at 22 kDa (A), with the specificity of the antibody confirmed using a recombinant PRR protein. Angiotensin II receptor 1 was detected in all three samples at the expected molecular weight of 41 kDa (B); Angiotensin II receptor 2 was detected in two out of three samples at 50 kDa (C); angiotensin converting enzyme was detected at a low abundance in all three samples at the expected molecular weight of 194 kDa (D); and β-actin confirmed approximate equivalent protein loading for all LMCA samples examined (E)