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Figure 1. RNA-Seq analysis of two primary breast tumors (PT1-2), one normal breast tissue sample (nBreast), three breast to brain metastatic tumors (MT1-3), and one normal brain tissue sample (nBrain). A: Heatmap comparison of gene expression in each group, presented as log2-normalized fragments per kilobase of transcript per million mapped reads (FPKM). Colors indicate relatively low (blue) or high (red) gene expression; B: the principal component analysis (PCA) of log2-normalized FPKM values, showing similarities and differences between sample groups; C: protein-protein interactions of selected genes overexpressed in metastatic tissue (MT) samples compared to all other groups; D: real-time PCR quantification of genes overexpressed in different samples (n = 3, P < 0.05). Error bars indicate SEM